abstract：:The concept of pilot pseudopodia is reconsidered 30 years after its inauguration (Gerisch, G., Hülser, D., Malchow, D., Wick, U., 1975. Cell communication by periodic cyclic-AMP pulses. Phil. Trans. R. Soc. Lond. B 272, 181-192). The original hypothesis stated that protruding pseudopodia serve as dynamic sensory organ...

journal_title：European journal of cell biology

authors： Weber I

更新日期：2006-09-01 00:00:00

abstract：:Macrocyst formation in the cellular slime moulds is a sexual process induced under dark and humid conditions. Normal development life cycle in these organisms involves proliferation by cell division and, upon starvation, formation of multicellular aggregates and fruiting bodies, consisting of spores and stalk cells. M...

journal_title：European journal of cell biology

authors： Urushihara H,Muramoto T

更新日期：2006-09-01 00:00:00

abstract：:In the current study we describe the changes of overall organization of lens fiber cells in connexin 46 (Cx46) and connexin 50 (Cx50) knockout mice. Morphometric analyses and the application of immunocytochemical techniques revealed that in Cx46 knockout lens (Cx46 -/-), where Cx50 is expressed alone, the postnatal di...

journal_title：European journal of cell biology

authors： Dunia I,Cibert C,Gong X,Xia CH,Recouvreur M,Levy E,Kumar N,Bloemendal H,Benedetti EL

更新日期：2006-08-01 00:00:00

abstract：:Hyaluronan is a straight chain, glycosaminoglycan polymer of the extracellular matrix composed of repeating units of the disaccharide [-D-glucuronic acid-beta1,3-N-acetyl-D-glucosamine-beta1,4-]n. Hyaluronan is synthesized in mammals by at least three synthases with products of varying chain lengths. It has an extraor...

journal_title：European journal of cell biology

authors： Stern R,Asari AA,Sugahara KN

更新日期：2006-08-01 00:00:00

abstract：:RNA interference (RNAi) is a powerful tool for functional genetic studies in model organisms and mammalian cells. To facilitate rapid construction of gene knockdown constructs and RNAi libraries for known genes of mammalian cells, a new and simple strategy to produce small interfering RNA (siRNA) expression vectors wi...

journal_title：European journal of cell biology

authors： Jian R,Peng T,Deng S,Jiang J,Hu F,An J,Cheng X

更新日期：2006-05-01 00:00:00

abstract：:Initial integrin-mediated cell-matrix adhesions (focal complexes) appear underneath the lamellipodia, in the regions of the "fast" centripetal flow driven by actin polymerization. Once formed, these adhesions convert the flow behind them into a "slow", myosin II-driven mode. Some focal complexes then turn into elongat...

journal_title：European journal of cell biology

authors： Bershadsky AD,Ballestrem C,Carramusa L,Zilberman Y,Gilquin B,Khochbin S,Alexandrova AY,Verkhovsky AB,Shemesh T,Kozlov MM

更新日期：2006-04-01 00:00:00

abstract：:Activation of protein kinase C by phorbol esters triggers the remodelling of the actin cytoskeleton and the formation of podosomes in smooth muscle cells (SMCs). Regional control of actin dynamics at specialised microdomains results in a local reduction in contractile forces. The molecular basis for this local inhibit...

journal_title：European journal of cell biology

authors： Lener T,Burgstaller G,Crimaldi L,Lach S,Gimona M

更新日期：2006-04-01 00:00:00

abstract：:Integrin-linked kinase (ILK) represents a key component of integrin signaling complexes that functions in concert with multiple binding partners to transmit cues from the extracellular matrix environment to the actin cytoskeleton. Both gain- and loss-of-function approaches to study ILK have confirmed the essential rol...

journal_title：European journal of cell biology

authors： Boulter E,Van Obberghen-Schilling E

更新日期：2006-04-01 00:00:00

abstract：:Among sarcomeric muscles the cardiac muscle cells are unique by, inter alia, a systemic and extended cell-cell contact structure, the intercalated disk (ID), comprising frequent and closely spaced arrays of plaque-coated cell-cell adhering junctions (AJs). As some of these junctions may look somewhat like desmosomes a...

journal_title：European journal of cell biology

authors： Franke WW,Borrmann CM,Grund C,Pieperhoff S

更新日期：2006-02-01 00:00:00

abstract：:The transport of the chitin synthase III, Chs3p, to the plasma membrane is temporally and spatially regulated. Chs3p is delivered to the plasma membrane at the beginning of the cell cycle, forming chitin rings, and at the end of the cell cycle, forming the primary septum. During the rest of the cell cycle, it is maint...

journal_title：European journal of cell biology

authors： Ortiz D,Novick PJ

更新日期：2006-02-01 00:00:00

abstract：:To get new insights into the function of the intermediate filament (IF) protein vimentin in cell physiology, we generated two mutant cDNAs, one with a point mutation in the consensus motif in coil1A (R113C) and one with the complete deletion of coil 2B of the rod domain. In keratins and glia filament protein (GFAP), a...

journal_title：European journal of cell biology

authors： Schietke R,Bröhl D,Wedig T,Mücke N,Herrmann H,Magin TM

更新日期：2006-01-01 00:00:00

abstract：:Interleukin (IL)-6 is an important cytokine in inflammatory processes, differentiation and growth. The IL-6 receptor complex comprises the specific IL-6 receptor (gp80) and two molecules of the signal tranducing component gp130 which transduces the signal into the nucleus via the Jak-STAT pathway. Both, gp80 and gp130...

journal_title：European journal of cell biology

authors： Buk DM,Renner O,Graeve L

更新日期：2005-10-01 00:00:00

abstract：:Mutations in the LMNA gene, which encodes nuclear lamins A and C by alternative splicing, can give rise to Emery-Dreifuss muscular dystrophy. The mechanism by which lamins A and C separately contribute to this molecular phenotype is unknown. To address this question we examined ten LMNA mutations exogenously expressed...

journal_title：European journal of cell biology

authors： Motsch I,Kaluarachchi M,Emerson LJ,Brown CA,Brown SC,Dabauvalle MC,Ellis JA

更新日期：2005-09-01 00:00:00

abstract：:In the cell cycle the transition from G2 phase to cell division (M) is strictly controlled by protein phosphorylation-dephosphorylation reactions effected by several protein kinases and phosphatases. Although much indirect and direct evidence point to a key role of protein phosphatase 2A (PP2A) at the G2/M transition,...

journal_title：European journal of cell biology

authors： Klingler-Hoffmann M,Barth H,Richards J,König N,Kinzel V

更新日期：2005-08-01 00:00:00

abstract：:During maize pollen embryogenesis, a range of multicellular structures are formed. Using different approaches, the "nature" of these structures has been determined in terms of their embryogenic potential. In situ molecular identification techniques for gene transcripts and products, and a novel cell tracking system in...

journal_title：European journal of cell biology

authors： Massonneau A,Coronado MJ,Audran A,Bagniewska A,Mòl R,Testillano PS,Goralski G,Dumas C,Risueño MC,Matthys-Rochon E

更新日期：2005-07-01 00:00:00

abstract：:Keratins I and II form the largest subgroups of mammalian intermediate filament (IF) proteins and account as obligatory heteropolymers for the keratin filaments of epithelia. All human type I genes except for the K18 gene are clustered on chromosome 17q21, while all type II genes form a cluster on chromosome 12q13, th...

journal_title：European journal of cell biology

authors： Zimek A,Weber K

更新日期：2005-06-01 00:00:00

abstract：:In the present study the role of endothelin (ET) and its receptors (ETA-R and ETB-R) in cellular mechanisms underlying the resistance of astroglial cells to low oxygen level and development of hypoxia has been investigated. To define the influences of ET and its receptors on survival and on antigenic as well as morpho...

journal_title：European journal of cell biology

authors： Danielyan L,Gembizki O,Proksch B,Weinmann M,Morgalla M,Wiesinger H,Buniatian GH,Gleiter CH

更新日期：2005-06-01 00:00:00

abstract：:Next to the already available mouse monoclonal and laboratory animal-derived polyclonal antibodies, recombinant antibodies offer an additional and virtually unlimited arsenal of new immunohistochemical research tools. The major advantages of recombinant antibodies are their rapid and easy generation against virtually ...

journal_title：European journal of cell biology

authors： Raats JM,Hof D

更新日期：2005-04-01 00:00:00

abstract：:The linkage of the different types of cytoskeletal proteins to cell adhesion structures at the cytoplasmic membrane and the connection of these contact sites to corresponding sites of adjacent cells is a prerequisite for integrity and stability of cells and tissues. The structurally most prominent types of such cell-c...

journal_title：European journal of cell biology

authors： Schmidt A,Jäger S

更新日期：2005-03-01 00:00:00

abstract：:It has only recently been recognized that intermediate filaments (IFs) and their assembly intermediates are highly motile cytoskeletal components with cell-type- and isotype-specific characteristics. To elucidate the cell-type-independent contribution of actin filaments and microtubules to these motile properties, flu...

journal_title：European journal of cell biology

authors： Wöll S,Windoffer R,Leube RE

更新日期：2005-03-01 00:00:00

abstract：:Cancer/testis antigens (CTAs) are characterized by their restricted expression pattern. In normal individuals their expression is largely restricted to the testis. In the case of cancer patients, CTA expression has also been frequently observed in the tumoral cells. CTAs are considered to be promising targets for immu...

journal_title：European journal of cell biology

authors： Alsheimer M,Drewes T,Schütz W,Benavente R

更新日期：2005-03-01 00:00:00

abstract：:In the literature the question of whether a system structurally and functionally related to the barrier function of the tight junctions (TJs) of polarized epithelia exists in the epidermis has been and still is controversially discussed. We have systematically addressed this question in a study of the granular layer o...

journal_title：European journal of cell biology

authors： Schlüter H,Wepf R,Moll I,Franke WW

更新日期：2004-12-01 00:00:00

abstract：:Dermatological diseases range from minor cosmetic problems to life-threatening conditions, as seen in some severe disorders of keratinization and cornification. These disorders are commonly due to abnormal epidermal differentiation processes, which result in disturbed barrier function of human skin. Elucidation of the...

journal_title：European journal of cell biology

authors： Zeeuwen PL

更新日期：2004-12-01 00:00:00

abstract：:Centaurin alpha-1 is a high-affinity PtdIns(3,4,5)P3-binding protein enriched in brain. Sequence analysis indicates centaurin alpha-1 contains two pleckstrin homology domains, ankyrin repeats and an Arf GAP homology domain, placing it in the AZAP family of phosphoinositide-regulated Arf GAPs. Other members of this fam...

journal_title：European journal of cell biology

authors： Thacker E,Kearns B,Chapman C,Hammond J,Howell A,Theibert A

更新日期：2004-10-01 00:00:00

abstract：:LIM kinase (LIMK) plays a critical role in stimulus-induced remodeling of the actin cytoskeleton by linking signals from the Rho family GTPases to changes in cofilin activity. Recent studies have shown an important role for LIMK1 signaling in tumor cell invasion through regulating actin dynamics. In this study, we inv...

journal_title：European journal of cell biology

authors： Nishimura Y,Yoshioka K,Bernard O,Himeno M,Itoh K

更新日期：2004-08-01 00:00:00

abstract：:To study molecules secreted from cultured plant cells that promote development, maize microspores were transferred into culture and the conditioned media were collected over time and analysed. Electrophoresis indicated that both non-glycosylated and glycosylated proteins including arabinogalactan proteins (AGPs) appea...

journal_title：European journal of cell biology

authors： Borderies G,le Béchec M,Rossignol M,Lafitte C,Le Deunff E,Beckert M,Dumas C,Elisabeth MR

更新日期：2004-07-01 00:00:00

abstract：:The AtT20 pituitary cell is the one that was originally used to define the pathways taken by secretory proteins in mammalian cells. It possesses two secretory pathways, the constitutive for immediate secretion and the regulated for accumulation and release under hormonal stimulation. It is in the regulated pathway, mo...

journal_title：European journal of cell biology

authors： Daull P,Home W,LeBel D

更新日期：2004-04-01 00:00:00

abstract：:Alternative splicing in the extracellular domain is a characteristic feature of members of the fibroblast growth factor receptor (FGFR) family. This splicing event generates receptor variants, which differ in their ligand binding specificities. A poorly characterized splice variant is FGFR1-IIIb, recently found to be ...

journal_title：European journal of cell biology

authors： Zhang H,Dessimoz J,Beyer TA,Krampert M,Williams LT,Werner S,Grose R

更新日期：2004-02-01 00:00:00

abstract：:Evolution of neoplastic cells has generally been regarded as a cumulative intrinsic process resulting in altered cell characteristics enabling enhanced growth properties, evasion of apoptotic signals, unlimited replicative potential and gain of properties enabling the ability to thrive in ectopic tissues and in some c...

journal_title：European journal of cell biology

authors： van Kempen LC,Ruiter DJ,van Muijen GN,Coussens LM

更新日期：2003-11-01 00:00:00

abstract：:Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described ...

journal_title：European journal of cell biology

authors： Verschuure P,Tatard C,Boelens WC,Grongnet JF,David JC

更新日期：2003-10-01 00:00:00

abstract：:The spatial and temporal distribution as well as ultrastructural and biochemical characteristics of apoptotic and mitotic cells during human eye development were investigated in 14 human conceptuses of 4-9 postovulatory weeks, using electron and light microscopy. In the 5th developmental week, apoptotic and mitotic ce...

journal_title：European journal of cell biology

authors： Bozanić D,Tafra R,Saraga-Babić M

更新日期：2003-08-01 00:00:00

abstract：:The P446L mutant Drosophila importin-beta (P446L-imp-beta) has been reported to prohibit--in dominant negative fashion--nuclear envelope (NE) assembly. Along elucidating the mode of action of P446L-imp-beta we studied in vitro NE assembly on Sepharose beads. While Drosophila embryo extracts support NE assembly over Se...

journal_title：European journal of cell biology

authors： Tirián L,Timinszky G,Szabad J

更新日期：2003-07-01 00:00:00

abstract：:Plastids, an essential group of plant cellular organelles, proliferate by division to maintain continuity through cell lineages in plants. In recent years, it was revealed that the bacterial cell division protein FtsZ is encoded in the nuclear genome of plant cells, and plays a major role in the plastid division proce...

journal_title：European journal of cell biology

authors： Momoyama Y,Miyazawa Y,Miyagishima SY,Mori T,Misumi O,Kuroiwa H,Tsuneyoshi K

更新日期：2003-06-01 00:00:00

abstract：:Involvement of CDK2 in glucocorticoid-mediated S-phase lengthening was analyzed in this work. Dexamethasone (DXM) treatment of PHA-stimulated lymphocytes induced a decrease in CDK2 mRNA expression without any change in mRNA stability. This glucocorticoid-induced decrease in CDK2 mRNA expression could be suppressed by ...

journal_title：European journal of cell biology

authors： Peiretti A,Baghdassarian N,Gerland LM,Ffrench P,Bryon PA,Magaud JP,Ffrench M

更新日期：2003-05-01 00:00:00

abstract：:Human ECV304 cells respond reproducibly by tube formation to complex basement membrane matrices. Laminins are major glycoproteins of basement membranes. We therefore studied the ability of ECV304 cells to attach to defined laminin isoforms and to fibronectin, and identified the involved laminin receptors. The cells bo...

journal_title：European journal of cell biology

authors： Genersch E,Ferletta M,Virtanen I,Haller H,Ekblom P

更新日期：2003-03-01 00:00:00

abstract：:The transcriptional status of cells can be deduced from the staining pattern of various nuclear markers such as the Cajal body, nucleolus and nuclear speckles. In this study we have used these markers to correlate transcriptional status with cell differentiation in the lens. As a closed system with no cell loss and wi...

journal_title：European journal of cell biology

authors： Gribbon C,Dahm R,Prescott AR,Quinlan RA

更新日期：2002-10-01 00:00:00

abstract：:Gap junctions are known to play a role in the control of cell proliferation, and connexins (Cx) are considered to be tumor suppressors. However, the effects of Cx on cell proliferation are dependent on the Cx which is expressed and on the cell type under consideration. We previously found that restoration of cell-to-c...

journal_title：European journal of cell biology

authors： Flachon V,Tonoli H,Selmi-Ruby S,Durand C,Rabilloud R,Rousset B,Munari-Silem Y

更新日期：2002-05-01 00:00:00

abstract：:We describe here the cloning, expression and characterisation of centaurin-alpha2 from a rat adipocyte cDNA library. The centaurin-alpha2 cDNA contains an open reading frame, which codes for a protein of 376 amino acids with predicted mass of 43.5 kDa. Centaurin-alpha2 shares 51-59% identity with centaurin-alpha1 prot...

journal_title：European journal of cell biology

authors： Whitley P,Gibbard AM,Koumanov F,Oldfield S,Kilgour EE,Prestwich GD,Holman GD

更新日期：2002-04-01 00:00:00

abstract：:Tubular aggregates are specific subcellular structures that appear in skeletal muscle fibres under different pathological conditions. The origin of the tubular aggregates is generally ascribed to proliferating membranes of sarcoplasmic reticulum. There are, however, histochemical indications for the presence of mitoch...

journal_title：European journal of cell biology

authors： Novotová M,Zahradník I,Brochier G,Pavlovicová M,Bigard X,Ventura-Clapier R

更新日期：2002-02-01 00:00:00

abstract：:SGLT1, an isoform of Na+-dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. To search the domain responsible for the apical localization of ...

journal_title：European journal of cell biology

authors： Suzuki T,Fujikura K,Koyama H,Matsuzaki T,Takahashi Y,Takata K

更新日期：2001-12-01 00:00:00